exosomes isolated from a normal human adult dermal fibroblast cell line (Lonza)
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exosomes isolated from a normal human adult dermal fibroblast cell line
Exosomes Isolated From A Normal Human Adult Dermal Fibroblast Cell Line, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exosomes+isolated+from+a+normal+human+adult+dermal+fibroblast+cell+line/exosomes+isolated+from+a+normal+human+adult+dermal+fibroblast+cell+line/pmc06224575-227-0-11
Average 90 stars, based on 1 article reviews
Exosomes Isolated From A Normal Human Adult Dermal Fibroblast Cell Line, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exosomes+isolated+from+a+normal+human+adult+dermal+fibroblast+cell+line/exosomes+isolated+from+a+normal+human+adult+dermal+fibroblast+cell+line/pmc06224575-227-0-11
Average 90 stars, based on 1 article reviews
exosomes isolated from a normal human adult dermal fibroblast cell line - by Bioz Stars,
2026-11
90/100 stars
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1) Product Images from "Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling"
Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling
Journal: Scientific Reports
doi: 10.1038/s41598-018-34879-6
Figure Legend Snippet: Characterization of isolated exosomes. ( a ) Electron microscopy images reveal WT- and Dys-iCM secreted exosomes display traditional cuplike morphology and are approximately 50 nm. ( b ) NTA of isolated exosomes reveals a range of sizes in particles averaging 148 nm (WT-exo) and 187 (Dys-exo). ( c) Quantitation of NTA results. ( d) Exosomes exhibit exosomal markers CD63 and CD81 as shown by flow cytometry. ( e ) Exo-Check protein array analysis reveals WT- and Dys-exo display exosome protein markers. ( f ) Cardiomyocytes are labeled with NCX1-eGFP and exosomes are stained with PKH26 (red). Exosomes are seen to be taken up at 2 hours.
Techniques Used: Isolation, Electron Microscopy, Quantitation Assay, Flow Cytometry, Protein Array, Labeling, Staining
Figure Legend Snippet: Exosomes protect against stress-induced cell injury in Dys-iCMs. Exposure to WT- and Dys-exos prior to stress ( a , b ) decreased ROS levels. Dermal fibroblast exos did not reduce stress induced ROS levels in Dys1-iCMs as significantly as iCM-derived exos. n = 3/group, *p < 0.05 vehicle stress vs. vehicle no stress, # p < 0.05 exosome exposure vs. vehicle stress.
Techniques Used: Derivative Assay
Related Articles
Isolation:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Control:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Electron Microscopy:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Quantitation Assay:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Flow Cytometry:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Protein Array:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Labeling:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Staining:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Derivative Assay:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Cell Culture:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Membrane:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Phospho-proteomics:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Blocking Assay:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Ab Array:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. Incubation:Article Title: Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling Article Snippet: Trypsin was inactivated with DMEM media (Thermo Fisher Scientific, Waltham, MA) with 10% fetal bovine serum. |